期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 568, 期 -, 页码 38-45出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2015.01.005
关键词
OmpD porin; Hydrogen peroxide; Hypochlorous acid; Homology modeling; Molecular simulations
资金
- FONDECYT [1120384, 11130576]
- Millenium Initiative [09-022-F]
OmpD is the major Salmonella enterica serovar Typhimurium (S. Typhimurium) porin and mediates hydrogen peroxide (H2O2) influx. The results described herein extend this finding to hypochlorous acid (HOCI), another reactive oxygen species that is also part of the oxidative burst generated by the phagosome. S. Typhimurium cells lacking OmpD show decreased HOCI influx, and OmpD-reconstituted proteoliposomes show an increase in the uptake of the toxic compound. To understand this physiologically relevant process, we investigated the role of key OmpD residues in H2O2 and NaOCl transport. Using a theoretical approach, residue K16 was defined as a major contributor to the channel electrostatic properties, and E1 II was shown to directly participate in the size-exclusion limit of the channel. Together, we provide theoretical, genetic, and biochemical evidence that OmpD mediates H2O2 and NaOCl uptake, and that key residues of the channel are implicated in this process. (C) 2015 Elsevier Inc. All rights reserved.
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