期刊
STEM CELLS AND DEVELOPMENT
卷 24, 期 3, 页码 312-319出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/scd.2014.0191
关键词
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资金
- Chinese government
- Shandong government
- Beijing government [National Natural Science Foundation of China] [81200758, 81470709]
- Beijing government [Independent Innovation Foundation of Shandong University] [2011GN038]
- Beijing government [Jinan Scientific Researchers Start-up Program of University] [201102066, 201302030]
- Beijing government [Shandong Provincial Natural Science Foundation] [2010GSF10267, ZR2011HQ017]
- Beijing government [Beijing Municipality Government ] [2013_014226_000055, PXM2014_014226_000048, PXM2014_014226_000013, PXM2014_014226_000053, Z121100005212004, PXM 2013_014226_000021, PXM 2013_014226_07_000080, TJSHG201310025005]
microRNAs (miRNAs) are short 20- to 22-nucleotide noncoding RNAs that negatively regulate the expression of target genes at the post-transcriptional level. The expression of specific miRNAs and their roles in the osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) exposed to mechanical stretch remain unclear. Here, we found that stretch induced both osteogenic differentiation and the differential expression of miR-21 in PDLSCs. Furthermore, we identified activin receptor type IIB (ACVR2B) as a target gene of miR-21. Luciferase reporter assays showed that miR-21 interacts directly with the 3 '-untranslated repeat sequence of ACVR2B mRNA. Mechanical stretch suppressed ACVR2B protein levels in PDLSCs, and this suppressive effect was modulated when endogenous miR-21 levels were either enhanced or inhibited. Both stretch and the expression of miR-21 altered endogenous ACVR2B protein levels and thus the osteogenic differentiation of PDLSCs. In addition, gain- and loss of function of ACVR2B mediated the osteogenic differentiation of PDLSCs. This study demonstrates that miR-21 is a mechanosensitive gene that plays an important role in the osteogenic differentiation of PDLSCs exposed to stretch.
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