4.6 Article Proceedings Paper

Production of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinamer

期刊

NEW BIOTECHNOLOGY
卷 46, 期 -, 页码 45-53

出版社

ELSEVIER
DOI: 10.1016/j.nbt.2018.07.001

关键词

Antimicrobial peptide; Elastin-Like recombinamer; Hepcidin; Antimicrobial activity

资金

  1. FCT I.P.
  2. ERDF through the COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI) [strategic program] [UID/BIA/04050/2013, POCI-01-0145-FEDER-007569]
  3. FCT within the ERA-NET IB, project FunBioPlas [ERA-IB-15-089]
  4. FCT within the ERA-NET IB, project FunBioPlas [FCT] [ERA-IB-2-6/0004/2014]
  5. DP_AEM
  6. FCT [PD/BD/113811/2015]
  7. Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) [NORTE-01-0145-FEDER-000009]
  8. Fundação para a Ciência e a Tecnologia [PD/BD/113811/2015, ERA-IB-2-6/0004/2014] Funding Source: FCT

向作者/读者索取更多资源

With the lack of new chemical antibiotics and increasing pathogen resistance to those available, new alternatives are being explored. Antimicrobial peptides (AMPs) with a broad range of effects, including antibacterial, antifungal, and antiviral actions, have emerged as one of the options. They can be produced by recombinant DNA technology, but the chromatographic methods used for peptide purification are expensive and time consuming. Here, we describe the design, production, purification and assessment of the antibacterial activity of the human peptide hepcidin, using an elastin-like recombinamer as fusion partner. The recombinant protein Hep-A200 was produced in Escherichia coli and purified by a non-chromatographic procedure, exploiting the thermal properties of the A200 elastin-like recombinamer. Recombinant Hep-A200 was found to retain antibacterial activity against Gram-positive and Gram-negative species.

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