期刊
NEW BIOTECHNOLOGY
卷 30, 期 2, 页码 153-158出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2012.11.003
关键词
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资金
- Boston University
- Sequenom
- National Cancer Institute [HHSN261200700031C]
- Knut and Alice Wallenberg Foundation
- FORMAS [2006-2856]
- Swedish Research Council for medicine [2007-2720]
- Swedish Research Council for Natural Sciences and Technology [2006-5168]
- European Community
Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.
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