期刊
NEW BIOTECHNOLOGY
卷 29, 期 3, 页码 379-386出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2011.07.002
关键词
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资金
- Japan Society for the Promotion of Science (JSPS)
- National Research Council of Thailand (NRCT)
- National Science and Technology Development Agency of Thailand (NSTDA)
- Japan Science and Technology Agency (JST)
Use of super strains exhibiting tolerance to high temperature, acidity and ethanol is a promising way to make ethanol production economically feasible. We describe here the breeding and performance of such a multiple-tolerant strain of Saccharomyces cerevisiae generated by a spore-to-cell hybridization technique without recombinant DNA technology. A heterothallic strain showing a high-temperature (41 degrees C) tolerant (Htg(+)) phenotype, a derivative from a strain isolated from nature, was crossed with a homothallic strain displaying high-ethanol productivity (Hep(+)), a stock culture at the Thailand Institute of Scientific and Technological Research. The resultant hybrid TJ14 displayed ability to rapidly utilize glucose, and produced ethanol (46.6 g/l) from 10% glucose fermentation medium at high temperature (41 degrees C). Not only ethanol productivity at 41 degrees C but also acid tolerance (Acd(+)) was improved in TJ14 as compared with its parental strains, enabling TJ14 to grow in liquid medium even at pH 3. TJ14 maintained high ethanol productivity (46.0 g/l) from 10% glucose when fermentation was done under multiple-stress conditions (41 degrees C and pH 3.5). Furthermore, when TJ14 was subjected to a repeated-batch fermentation scheme, the growth and ethanol production of TJ14 were maintained at excellent levels over ten cycles of fermentation. Thus, the multiple-stress (Htg(+) Hep(+) Acd(+)) resistant strain TJ14 should be useful for cost-effective bioethanol production under high-temperature and acidic conditions.
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