期刊
NEW BIOTECHNOLOGY
卷 29, 期 3, 页码 395-401出版社
ELSEVIER
DOI: 10.1016/j.nbt.2011.11.006
关键词
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资金
- Fundacao para a Ciencia e a Tecnologia (Portugal) [SFRH/BD/32514/2006]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/32514/2006] Funding Source: FCT
In this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentative broth was proposed using a single activated charcoal column. The FOS adsorption onto the activated charcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25 degrees C. Mixtures containing 50.6% (w/w) of FOS (FOS content in the fermentative broth) were purified to 92.9% (w/w) with a FOS recovery of 74.5% (w/w). Moreover, with the proposed process, fractions with purity up to 97% (w/w) of FOS were obtained. This purification process was also found to be efficient in the desalting of the fermentative broth.
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