期刊
NEW BIOTECHNOLOGY
卷 28, 期 6, 页码 574-580出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2011.01.009
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To evaluate the feasibility of producing kefiran industrially, whey lactose, a by-product from dairy industry, was used as a low cost carbon source. Because the accumulation of lactic acid as a by-product of Lactobacillus kefiranofaciens inhibited cell growth and kefiran production, the kefir grain derived and non-derived yeasts were screened for their abilities to reduce lactic acid and promote kefiran production in a mixed culture. Six species of yeasts were examined: Torulaspora delbrueckii IFO 1626; Saccharomyces cerevisiae IFO 0216; Debaryomyces hansenii TISTR 5155; Saccharomyces exiguus TISTR 5081; Zygosaccharomyces rouxii TISTR 5044; and Saccharomyces carlsbergensis TISTR 5018. The mixed culture of L. kefiranofaciens with S. cerevisiae IFO 0216 enhanced the kefiran production best from 568 mg/L in the pure culture up to 807 and 938 mg/L in the mixed cultures under anaerobic and microaerobic conditions, respectively. The optimal conditions for kefiran production by the mixed culture were: whey lactose 4%; yeast extract 4%; initial pH of 5.5; and initial amounts of L. kefiranofaciens and S. cerevisiae IFO 0216 of 2.1 x 10(7) and 4.0 x 10(6) CFU/mL, respectively. Scaling up the mixed culture in a 2 L bioreactor with dissolved oxygen control at 5% and pH control at 5.5 gave the maximum kefiran production of 2580 mg/L in batch culture and 3250 mg/L in fed-batch culture.
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