期刊
NEW BIOTECHNOLOGY
卷 27, 期 4, 页码 347-351出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2010.02.007
关键词
-
资金
- Council for Scientific and Industrial Research
Aspergillus strain SA 58, showing considerable beta glucosidase production was selected as the potential strain. The fungus showed enzyme production in both acidic and alkaline pH. A temperature of 35 degrees C was found to be optimum for enzyme production. Maximum enzyme production was seen when pectin was used as the carbon source (80 U/ml). In solid-state fermentation, an enzyme production of 6200 U/g Initial Dry Substrate was noted. The strain produced two extra cellular enzymes and two intra cellular enzymes. For both the extra cellular enzymes (BGL A and BGL B), 60 degrees C was found to be optimum temperature for activity. BGL A showed an optimum pH of 4.0 while BGL B showed an optimum pH of 3.0 for activity. Both the enzymes showed a second peak of activity at pH 9.0. Both BGL A and BGL B showed high thermal stabilities with residual activities of 86% and 85% even after 6 h of incubation at 50 degrees C.
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