期刊
NEW BIOTECHNOLOGY
卷 27, 期 4, 页码 409-418出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2010.05.005
关键词
-
资金
- Council of Agriculture, Taiwan, R.O.C. [93AS-1.9.2-BQ-B1(1), 94AS-13.3.2-BQ-B1(6), 96AS-4.1.2-IC-I1(2), 97AS-4.1.2-IC-I1(6), 98AS-4.1.1-IC-I1(1)]
- National Science Council, Taiwan, R.O.C. [98-2313-B-005-025-MY3, 99-2622-B-005-006-CC2]
- Ministry of Education, Taiwan, R.O.C.
- National Chung Hsing University, Taiwan, R.O.C.
Fusarium wilt, caused by Fusarium oxysporum (Fo), is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, F. oxysporum f. sp. niveum (Fon) is the damaging pathogen causing Fusarium wilt disease on watermelon, whereas F. oxysporum f. sp. cubense is the pathogen that infects banana. A rapid and reliable pathogen identification or disease diagnosis is essential for the integrated disease management practices in many crops. In this study, two new primer sets, Fon-1/Fon-2 and FnSc-1/FnSc-2, were developed to differentiate Fon and Fo, respectively. The PCR method using the novel primer sets has high sensitivity to detect Fon when the DNA concentration was as low as 0.01 pg or when the conidia number was as few as 5. In comparison with the published primer set, the Fon-1/Fon-2 primer set, derived from the sequence of OP-M12 random primer-amplified fragment, produced a 174 bp DNA fragment, and was more specific to Fon in Taiwan. In addition, with optimized PCR parameters, the molecular method using the Fon-1/Fon-2 primer set could directly detect Fon even when watermelon samples were collected in its early stage of disease development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据