期刊
STEM CELLS
卷 33, 期 7, 页码 2232-2242出版社
WILEY-BLACKWELL
DOI: 10.1002/stem.2017
关键词
Fate specification; Neural stem cell; Regionalization; Transcriptome; Subventricular zone; Postnatal neurogenesis
资金
- Swiss National Research Project [NRP63, 406340_128291]
- Swiss National Fund [31003A_127082]
- Mexican Government (CONACYT)
- Forschungskredit from the University of Zurich [K-41211-01-01]
- Programme Avenir Lyon Saint-Etienne
- Deutsche Forschungsgemeinschaft [DFG SFB592, TA-310-1, TA-310-2]
- Max Planck Society
- Swiss National Science Foundation [310030_143767]
- Swiss National Science Foundation (SNF) [31003A_127082, 310030_143767, 406340_128291] Funding Source: Swiss National Science Foundation (SNF)
Throughout postnatal life in mammals, neural stem cells (NSCs) are located in the subventricular zone (SVZ) of the lateral ventricles. The greatest diversity of neuronal and glial lineages they generate occurs during early postnatal life in a region-specific manner. In order to probe heterogeneity of the postnatal SVZ, we microdissected its dorsal and lateral walls at different postnatal ages and isolated NSCs and their immediate progeny based on their expression of Hes5-EGFP/Prominin1 and Ascl1-EGFP, respectively. Whole genome comparative transcriptome analysis revealed transcriptional regulators as major hallmarks that sustain postnatal SVZ regionalization. Manipulation of single genes encoding for locally enriched transcription factors (loss-of-function or ectopic gain-of-function in vivo) influenced NSC specification indicating that the fate of regionalized postnatal SVZ-NSCs can be readily modified. These findings reveal the pronounced transcriptional heterogeneity of the postnatal SVZ and provide targets to recruit region-specific lineages in regenerative contexts. Stem Cells 2015;33:2232-2242
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