期刊
NEUROUROLOGY AND URODYNAMICS
卷 30, 期 4, 页码 606-611出版社
WILEY-BLACKWELL
DOI: 10.1002/nau.21045
关键词
bladder urothelial cells; bladder urothelium; overactive bladder; TRPV1 channel
资金
- NIH [R01-DK075728]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK075728] Funding Source: NIH RePORTER
Aims: To measure transient receptor potential vanilloid type 1 (TRPV1) signaling in human bladder urothelial cells (BUC) from non-neurogenic overactive bladder (OAB) patients and control subjects (NB) BUC. Materials and Methods: Primary BUC cell cultures were derived from cystoscopic biopsies from two OAB and two NB subjects. TRPV1 expression was detected by immunofluorescence, PCR and Western blot staining. TRPV1 function was assessed by capsaicin (CAP, 6 mu M)-evoked intracellular calcium ([Ca(2+)](i)) changes measured by microfluorimetry imaging. CAP evoked changes in inward and outward currents were recorded electrophysiologically using excised outside-out patches and whole cell configurations using various protocols. Results: OAB BUC had significantly increased expression of TRPV1 compared to NB BUC on Western blot. CAP evoked significantly higher maximal [Ca(2+)](i) change over baseline in OAB (84.71 +/- 8.96%) compared to NB BUC (60.32 +/- 7.93%) (P < 0.05). CAP induced significantly greater percent change in single channel open probability(205.94 +/- 20.53% OABvs. 141.26 +/- 16.53% NB, P < 0.05) and normalized inward currents (13.54 +/- 1.6 4 pA/pF OAB vs. 8.28 +/- 0.89 pA/pF NB, P < 0.05). CAP caused significantly higher percent increase from baseline of whole cell outward currents in OAB (177.12 +/- 44.46%) compared to NB BUC (135.98 +/- 44.28%) (P < 0.05). Similarly thermal stimulus (45C solution) evoked significantly higher percent increase in whole cell outward currents in OAB (183.93 +/- 14.07%) compared to NB (145.61 +/- 10.12%) BUC (P < 0.05). These responses were blocked by 10 mM capsazepine (CPZ), a TRPV1 antagonist. Conclusions: Because only a few subjects were studied, augmented TRPV1 signaling cannot be generalized to all OAB subjects. However, the findings are consistent with the hypothesis that BU Care involved in sensory signaling. Neurourol. Urodynam. 30:606-611, 2011. (C) 2011 Wiley-Liss, Inc.
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