期刊
NEUROTOXICOLOGY
卷 29, 期 3, 页码 466-470出版社
ELSEVIER
DOI: 10.1016/j.neuro.2008.02.012
关键词
manganese; nuclear fraction; mitochondria; Z310 cells; RBE4 cells; PC12 cells; N27 cells
资金
- NIEHS NIH HHS [R01 ES008146-10, R21 ES013118, R01 ES008146-11, ES 08164, R01 ES008146, ES 013118, R21 ES013118-02] Funding Source: Medline
Manganese (Mn) is known to pass across the blood-brain barrier and interact with dopaminergic neurons. However, the knowledge on the subcellular distribution of Mn in these cell types upon exposure to Mn remained incomplete. This study was designed to investigate the subcellular distribution of Mn in blood-brain barrier endothelial RBE4 cells, blood-cerebrospinal fluid barrier choroidal epithelial Z310 cells, mesencephalic dopaminergic neuronal N27 cells, and pheochromocytoma dopaminergic PC12 cells. The cells were incubated with 100 mu M MnCl2 with radioactive tracer Mn-54 in the culture media for 24 h. The subcellular organelles, i.e., nuclei, mitochondria, microsomes, and cytoplasm, were isolated by centrifugation and verified for their authenticity by determining the markers specific to cellular organelles. Data indicated that maximum Mn accumulation was observed in PC12 cells, which was 2.8, 5.2- and 5.9-fold higher than that in N27, Z310 and RBE4 cells, respectively. Within cells, about 92%, 72%, and 52% of intracellular Mn-54 were found to be present in nuclei of RBE4, Z310, and N27 cells, respectively. The recovery of Mn-54 in nuclei and cytoplasm of PC12 cells were 27% and 69%, respectively. Surprisingly, less than 0.5% and 2.5% of cellular Mn-54 was found in mitochondrial and microsomal fractions, respectively. This study suggests that the nuclei may serve as the primary pool for intracellular Mn; mitochondria and microsomes may play an insignificant role in Mn subcellular distribution. (C) 2008 Elsevier Inc. All rights reserved.
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