4.3 Article

Ca2+ channel currents in dorsal root ganglion neurons of P/Q-type voltage-gated Ca2+ channel mutant mouse, rolling mouse Nagoya

期刊

NEUROSCIENCE RESEARCH
卷 73, 期 3, 页码 199-206

出版社

ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2012.04.006

关键词

omega-Agatoxin IVA; omega-Conotoxin GVIA; Patch clamp; Ba2+ current; Ca(V)2.1

资金

  1. KAKENHI [16780200, 18380175]
  2. Grants-in-Aid for Scientific Research [22500396, 18380175, 16780200] Funding Source: KAKEN

向作者/读者索取更多资源

The role of the P/Q-type voltage-gated Ca2+ channels (VGCCs) in release of neurotransmitters involved in nociception is not fully understood. Rolling mouse Nagoya (tg(rol)), a P/Q-type channel mutant mouse, expresses P/Q-type VGCC whose activation curve has a higher half activation potential and a smaller slope factor than the wild type channel. We previously reported that tg(rol) mice showed hypoalgesic responses to noxious stimuli. In this study, we examined the VGCC current in dorsal root ganglion (DRG) neurons by the whole-cell patch-clamp method. Both omega-agatoxin IVA (0.1 mu M) and omega-conotoxin GVIA (1 mu M) inhibited the VGCC current by about 40-50% in both the homozygous tg(rol) (tg(rol)/tg(rol)) and wild type (+/+) mice. The voltage-activation relationships of the total VGCC current and the omega-agatoxin IVA-sensitive component in the tg(rol)/tg(rol) mice shifted positively compared to the +/+ mice, whereas that sensitive to the omega-conotoxin GVIA was not different between the two genotypes. The time constant of activation of the VGCC current at -20 mV was longer in the tg(rol)/tg(rol) mice than in the +/+ mice. These changes in the properties of the VGCC in the tg(rol)/tg(rol) mouse may reduce the amount of the released neurotransmitters and account for the hypoalgesic responses. (C) 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

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