期刊
NEUROSCIENCE LETTERS
卷 575, 期 -, 页码 1-6出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2014.05.038
关键词
6-OHDA; Substantia Nigra pars compacta (SNc); Dopaminergic neuron; N-type calcium channel; cAMP-Dependent protein kinase (PKA)
资金
- National Basic Research Program of China (973 Program) [2011CB510002]
- Key Program of National Natural Science Foundation of China [30930095]
Voltage gated calcium channels (VGCC) are sensitive to oxidative stress, and their activation or inactivation can impact cell death. Although these channels have been extensively studied in expression systems, their role in the brain, particularly in the substantia nigra pars compacta (SNc), remain controversial. In this study, we assessed 6-hydroxydopamine (6-OHDA) induced transformation of firing pattern and functional changes of calcium channels in SNc dopaminergic neurons. Application of 6OHDA (0.5-2 mM) evoked a dose-dependent, desensitizing inward current and intracellular free calcium concentration ([Ca2+](i)) rise. In voltage clamp, omega-conotoxin-sensitive Ca2+ current modulation mediated by 6-OHDA reflected an altered sensitivity. Furthermore, we found that 6-OHDA modulated Ca2+ currents through PICA pathway. These results provided evidence for the potential role of VGCCs and PKA involved in oxidative stress in degeneration of SNc neurons in Parkinson's disease (PD). (C) 2014 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据