期刊
NEUROSCIENCE LETTERS
卷 534, 期 -, 页码 123-127出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2012.11.052
关键词
Honokiol; Anti-inflammation; Ischemia-reperfusion; Primary glial cell; Nuclear factor-kappa B; Inflammatory factor
资金
- Chinese National Technology Graveness Special Purpose Fund [2009zx09102-146]
- Natural Science Foundation of China [61071002]
This study was designed to investigate the effects of honokiol, a neuroprotective agent, on cerebral edema in cerebral ischemia reperfusion (IR) mice and its mechanism of anti-inflammation. Honokiol (0.7-70 mu g/kg) significantly reduced brain water contents and decreased the exudation of Evans blue dye from brain capillaries in cerebral IR mice. Honokiol (0.1-10 mu M) significantly reduced the p65 subunit level of NF-kappa B in the nucleus of primary culture-microglia. It (0.01-10 mu M) evidently reduced nitric oxide (NO) level in the microglia culture medium and in the microglia and astrocytes coculture medium. Honokiol (0.01-10 mu M) significantly decreased the level of TNF-alpha in the microglia medium or coculture cell medium. Honokiol (10 mu M) decreased the level of Regulated upon Activation Normal T-cell Expressed and Secreted (RANTES/CCL5) protein in medium of microglia or astrocytes. In conclusion, Honokiol has a potent anti-inflammatory effect in cerebral ischemia-reperfusion mice and this effect might be attributed to its inhibition ability on the NF-kappa B activation, consequently blocking the production of inflammatory factors including: NO, tumor necrosis factor-alpha (TNF-alpha) and RANTES/CCL5 in glial cells. These results provide evidence for the anti-inflammatory effect of honokiol for the potential treatment of ischemic (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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