期刊
NEUROSCIENCE LETTERS
卷 555, 期 -, 页码 248-252出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2013.06.001
关键词
Alkalinity; Avoidance behavior; Caenorhabditis elegans; Chemotaxis; Nociceptor; TRPV channel
资金
- NIH National Center for Research Resources
High pH is a noxious stimulus to animals, and their ability to avoid dangerously alkaline pH is critical for survival. However, the means by which they sense high pH has not been determined. The nematode Caenorhabditis elegans (C elegans) avoids environmental pH above 10.5. In contrast, C elegans mutants with structurally, developmentally, and/or functionally abnormal sensory cilia fail to avoid high pH, suggesting that sensory neurons in the cilia participate in sensing. Genetic rescue of the mutants indicates that ASH polymodal sensory neurons play a vital role in the process. Consistently, specific laser ablation of ASH neurons made animals insensitive to high pH. Furthermore, avoidance assays of other mutants also indicated that transient receptor potential vanilloid type (TRPV) ion channels encoded by osm-9 and ocr-2 are involved in sensing. Indeed, genetic rescue of osm-9 mutants by specifically expressing OSM-9 in ASH showed that TRPV channels play an essential role in sensing of high pH. Ca2+ imaging in vivo also revealed that ASH neurons were activated by high pH stimulation, but ASH of osm-9 or ocr-2 mutants were not. These results demonstrate that in C elegans, high pH is sensed by ASH nociceptors through opening of OSM-9/OCR-2 TRPV channels. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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