期刊
NEUROSCIENCE LETTERS
卷 473, 期 1, 页码 56-61出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2010.02.022
关键词
Amyotrophic lateral sclerosis; Spinal cord cultures; Astrocytes; Morphological transformation; GFAP; S100 beta
资金
- NIMHANS
We have earlier shown that cerebrospinal fluid (CSF) of amyotrophic lateral sclerosis (ALS) patients' produces selective degeneration of motor neurons, both in vitro as well as in vivo. The present study further evaluates the effect of ALS-CSF on the astrocytes in embryonic rat spinal cord cultures. We quantified the number of flat and process-bearing astrocytes in spinal cord cultures exposed to ALS-CSF and compared them against controls. In addition, GFAP and S100 beta expression were quantified by Western blot and measurement of immunofluorescence intensity respectively. We found higher number of process-bearing astrocytes in the cultures exposed to ALS-CSF. Both these proteins increased significantly in cultures exposed to ALS-CSF. Our results provide evidence that astroglia respond to toxic factor(s) present in ALS-CSF by undergoing morphological transformation from flat to process bearing which is further confirmed by elevated expression of GFAP and S100 beta. The above changes could possibly alter the microenvironment hastening the motor neuron degeneration. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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