期刊
NEUROSCIENCE LETTERS
卷 450, 期 1, 页码 23-26出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2008.10.108
关键词
Apoptosis; DNA strand breaks; Neuroprotection; Neuronal death
资金
- Ministerio de Education y Ciencia [SAF2006-05705]
- Ministerio de Sanidad y Consumo [PNSD SA002A07]
- Junta de Castilla y Leon
- Centre for Regenerative Medicine and Cell Therapy of Castilla y Leon,
- Federacion de Cajas de Ahorro de Castilla y Leon
Human serum albumin (HSA) is an effective therapeutic agent that protects neurons after cerebral ischemia or related injuries by means of its antioxidant capacity. Our aim was to test whether bovine serum albumin (BSA) might also provide protection, especially against DNA damage. Rat cortical neurons were cultured in both the presence and absence of BSA. To test the neuroprotective role of BSA against DNA damage and neuronal death, primary cultures were investigated using both gamma-H2AX and pATM immuno-cytochemistry, and the TUNEL assay, respectively. Quantitative analyses revealed that the cultures in the absence of BSA had a higher number of apoptotic neurons. Additionally, neurons showing DNA strand breaks were fewer when BSA was added to the medium. BSA acts as a neuroprotective molecule, reducing both the DNA damage and apoptosis rates. This effect is similar to that described for HSA, probably due to its antioxidant activity. Hence, we have demonstrated that BSA provides a neuroprotective role when DNA damage occurs. Additionally, we Suggest that BSA probably shares similarities with HSA in its antioxidant activity, opening new ways in the Study of stroke and related brain diseases. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
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