4.4 Article

DJ-1 mRNA anatomical localization and cell type identification in the mouse brain

期刊

NEUROSCIENCE LETTERS
卷 465, 期 3, 页码 214-219

出版社

ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2009.09.024

关键词

Parkinson's disease; In situ hybridization; Knockout mice

资金

  1. National Institute of Neurological Disorders and Stroke [R03NS058415, R01NS053919]
  2. American Parkinson Disease Association Center Grant
  3. American Parkinson Disease Association Research Grant

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Mutations in DJ-I cause familial Parkinson's disease (PD). The expression pattern of DJ-1 in the brain remains controversial. In the present study, we used DJ-I deficient mice as negative controls and examined DJ-1 mRNA expression in mouse brains. In sequential double labeling on the same sections, in situ hybridization of DJ-1 mRNA was followed by immunofluorescence detection of cell type markers. We found that DJ-I mRNA was expressed in the majority of neurons in all brain areas examined In particular, all dopamine neurons in the ventral midbrain expressed DJ-1 mRNA. In contrast, the choroid plexus and ependymal cells lining the ventricles were the only non-neuronal regions strongly expressing DJ-1 mRNA. DJ-I mRNA was not detected in astrocytes. The fact that DJ-1 mRNA is expressed in all nigra dopamine neurons but not in astrocytes suggests that its potential neuroprotective role could be cell-autonomous. Moreover, that DJ-1 expression is not restricted to substantia nigra dopamine neurons suggests that PD-linked mutant DJ-I may interact with other predisposing factors to cause the relatively selective dopamine neuron degeneration in Parkinson's disease. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

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