期刊
NEUROSCIENCE LETTERS
卷 465, 期 2, 页码 177-180出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2009.08.026
关键词
C2C12 cells; Loperamide; mu-Opioid receptors; Protein kinase C-zeta
资金
- China Medical University [CMU96-235]
The present Study is designed to investigate the role of atypical protein kinase C(PKC) in the signaling of mu-opioid receptors (MOR) for glucose uptake in myoblast C2C12 cells Loperamide enhanced the uptake of radioactive deoxyglucose into C2C12 Cells in a concentration-dependent manner that was abolished in cells pre-incubated with GF109203X at concentrations sufficient to block PKC Inhibition of the atypical zeta (zeta) isoform of PKC using myristoylated PKC pseudosubstrate resulted in a concentration-dependent decrease of loperamide-stimulated glucose uptake into C2C12 cells In addition, loperamide elicited the phosphorylation of PKC-zeta in C2C12 cells in a concentration-dependent manner that was abolished by pretreatment with naloxonazine at concentrations sufficient to block MOR. These results suggest the mediation of PKC-zeta in MOR signaling for glucose uptake in C2C12 cells. Activation of PKC-zeta by MOR stimulation is highly relevant to the search for therapeutic targets for glucose transport in insulin-sensitive tissues (c) 2009 Elsevier Ireland Ltd. All rights reserved
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