期刊
NEUROSCIENCE LETTERS
卷 441, 期 2, 页码 163-166出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2008.06.030
关键词
oncostatin M; rat brain endothelial cell; blood-brain barrier; tight junction; claudin-5; ZO-1
资金
- JSPS, Japan [(B) 17390159, 188902271, (B) 197901991]
- Ministry of Health, Labor and Welfare of Japan [H19-nanchi-ippan-006]
- Fukuoka University
- Grants-in-Aid for Scientific Research [17390159] Funding Source: KAKEN
Oncostatin M (OSM), a member of the interleukin-6 family, is produced by monocytes and macrophages in the peripheral blood and microglia in the brain. The present study aimed to elucidate a regulatory role of OSM in the functions of blood-brain barrier (BBB) comprised of rat brain capillary endothelial cells (RBECs). OSM decreased the transendothelial electrical resistance of RBEC monolayers in a concentration-and time-dependent manner. Immunocytochemical observations of ZO-1 and claudin-5 in OSM-treated RBECs showed a zipper-like and/or zigzag shape along the junctions between cells, in contrast with the smooth and linear shape in vehicle-treated cultures. When RBECs were pre-treated with anti-OSM antibody, OSM failed to evoke these changes. The cellular constituents producing OSM in the brain and peripheral blood could be implicated in the functional and structural impairment of the BBB. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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