期刊
NEUROSCIENCE
卷 186, 期 -, 页码 110-119出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2011.04.035
关键词
microdialysis; large molecule; beta-amyloid; inflammatory cytokine; in vivo sampling
资金
- Japan Promotion of Science
- Japanese Ministry of Education, Culture, Sports, Science and Technology
- Japan Science and Technology Agency
- Mitsubishi Pharma Research Foundation
- Grants-in-Aid for Scientific Research [11J05675] Funding Source: KAKEN
Microdialysis is a powerful in vivo technique for the continuous sampling of small molecules within the extracellular fluid space. However, efforts to collect larger molecules have met with little success. To identify biologically active larger molecules in free-moving animals would be of great benefit. For this purpose, we have developed a novel microdialysis method that allows consistent recovery of large molecules from the brain interstitial space in the awake, free-moving mouse. Using a new vent probe with a push-pull perfusion system, the present study successfully demonstrated in vivo sampling of pathophysiologically important macromolecules in free-moving mouse brain. This sampling system allowed monitoring of the dynamic changes in their concentrations. Overall, this novel microdialysis system would provide the opportunity to identify the expression patterns of pathophysiologically important proteins in a variety of physiological and pathological processes for a better understanding of various diseases. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
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