期刊
NEUROSCIENCE
卷 168, 期 3, 页码 778-786出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2010.04.019
关键词
Alzheimer's disease; amyloid beta; L-theanine; calcium; nitric oxide; neuroprotection
资金
- National Natural Science Foundation of China [30870587, 30871260]
- Natural Science Foundation of Hebei Province [C2007000251]
- Specialized Research Fund for the Doctoral Program of Higher Education [20070094004]
- Department of Science and Technology of China [2006CB500700]
- Key Laboratory of Mental Health, Chinese Academy of Sciences
As a natural analogue of glutamate, L-theanine is the unique amino acid derivative in green tea. Although its underlining mechanisms are not yet clear, it has been suggested that L-theanine treatment may prove beneficial to patients with neurodegenerative diseases. In this study, we investigated the neuroprotective effect and its mechanism of L-theanine in an in vitro model of Alzheimer's disease by using the human APP (Swedish mutation) transgenic SH-SY5Y cell. Amyloid beta (A beta) neurotoxicity was triggered by L-glutamate in this cell line. Additionally, L-theanine significantly attenuated L-glutamate-induced apoptosis at similar levels to those seen with the NMDA receptor inhibitor MK-801 in the stably expressing APP Swedish mutation SH-SY5Y cells which over-generated A beta. Meanwhile, the activation of c-Jun N-terminal kinase and caspase-3 induced by L-glutamate was suppressed by L-theanine. We also found that cells treated with L-theanine showed decreased production of nitric oxide resulting from the down-regulated protein levels of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS). These results indicate that the inhibition of the NMDA subtype of glutamate receptors and its related pathways is the crucial point of the neuroprotective effect of L-theanine in the cell model. Thus, our present study supports the notion that L-theanine may provide effective prophylaxis and treatment for Alzheimer's disease. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据