期刊
NEUROSCIENCE
卷 169, 期 3, 页码 1473-1485出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2010.04.058
关键词
neural stem cell markers; early progenitor cell markers; adult neural stem cells; embryonic neural stem cells; neurospheres
资金
- Spanish Ministry of Science and Innovation [SAF2006-4826, SAF2009-07974]
- Spanish Ministry of Health [RD06/0010/1010]
- Comunidad de Madrid [P-2006/BIO-204]
- CIBASA
- Spanish National Research Council [PIF200620F0041]
Two monoclonal antibodies (Nilo1 and Nilo2) were generated after immunization of hamsters with E13.5 olfactory bulb-derived mouse neurospheres. They are highly specific for neural stem and early progenitor cell surface antigens. Nilo positive cells present in the adult mouse subventricular zone (SVZ) were able to initiate primary neural stem cell cultures. Moreover, these antibodies added to neuro-sphere cultures induced proliferation arrest and interfered with their differentiation. In the lateral ventricles of adult mice, Nilo1 stained a cell subpopulation lining the ventricle and cells located in the SVZ, whereas Nilo2 stained a small population associated with the anterior horn of the SVZ at the beginning of the rostral migratory stream. Co-staining of Nilo1 or Nilo2 and neural markers demonstrated that Nilo1 identifies an early neural precursor subpopulation, whereas Nilo2 detects more differentiated neural progenitors. Thus, these antibodies identify distinct neurogenic populations within the SVZ of the lateral ventricle. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
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