期刊
NEUROSCIENCE
卷 163, 期 2, 页码 561-570出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2009.06.045
关键词
Alzheimer's disease; oligomeric A beta; cell culture; okadaic acid; TGF beta
资金
- James Tudor Foundation
- Alzheimer's Research Trust
Transforming growth factors beta (TGF beta) regulate multiple biological activities. TGF beta activation of the Smad pathway results in activation of genes encoding extracellular matrix molecules, proteases, protease activators and protease inhibitors. In Alzheimer's disease (AD), TGF beta protein and mRNA levels are raised, which would be expected to be neuroprotective. However, recent observations suggest that TGF beta-Smad signalling is disrupted by the hyperphosphorylation of tau, the primary component of neurofibrillary tangles: phosphorylated Smad2/3 (pSmad 2/3) co-localises with phosphorylated tau in the neuronal cytoplasm and levels are reduced in the nucleus. We have investigated whether in vitro induction of tau hyperphosphorylation influences pSmad 2/3 localisation in rat primary cortical cells. Treatment with okadaic acid, a protein phosphatase 1 and 2A inhibitor caused hyperphosphorylation of tau at epitopes hyperphosphorylated in AD and disrupted pSmad 2/3 translocation into the nucleus. The disruptive effect of tau phosphorylation on pSmad 2/3 translocation was confirmed by treatment of primary cortical cells with synthetic oligomeric A beta(1-42), a more physiologically relevant model of AD. Our findings suggest that despite the increased level of TGF beta in AD, the TGF beta-Smad signalling pathway is impeded within neurones due to sequestration of pSmad 2/3 by hyperphosphorylated tau. This may compromise neuroprotective actions of TGF beta and contribute to neurodegeneration in AD. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.
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