期刊
NEUROREPORT
卷 21, 期 6, 页码 447-451出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0b013e328338ba00
关键词
adeno-associated virus; gene transfer; marmoset; motor cortex; neuron; nonhuman primate; striatum; substantia nigra; tropism
资金
- JSPS
- PRESTO, JST
Although the adeno-associated virus (AAV) vector is a promising tool for gene transfer into neurons, especially for therapeutic purposes, neurotropism in primate brains is not fully elucidated for specific AAV serotypes. Here, we injected AAV serotype 8 (AAV8) vector carrying the enhanced green fluorescent protein (EGFP) gene under a ubiquitous promoter into the cerebral cortex, striatum and substantia nigra of common marmosets. Robust neuronal EGFP expression was observed at all injected sites. Cell typing with immunohistochemistry confirmed efficient AAV8-mediated gene transfer into the pyramidal neurons in the cortex, calbindin-positive medium spiny neurons in the striatum and dopaminergic neurons in the substantia nigra. The results indicate a preferential tropism of AAV8 for subsets of neurons, but not for glia, in monkey brains. NeuroReport 21: 447-451 (C) 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins.
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