期刊
NEUROREPORT
卷 20, 期 8, 页码 798-803出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0b013e32832b8be4
关键词
astrocytes; cell printing; collagen hydrogel; freeform fabrication; neurons; tissue engineering
资金
- Focused Ultrasound Surgery Foundation
- Gerald J. and Dorothy R. Friedman Foundation
We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites. NeuroReport 20:798-803 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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