期刊
NEUROPSYCHOPHARMACOLOGY
卷 35, 期 4, 页码 893-903出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/npp.2009.181
关键词
neurogenesis; neural progenitors; TPH; serotonin; brain; hippocampus
资金
- Deutsche Forschungsgemeinschaft (DFG) [Be2530]
- University of Munich
- [SFB 531]
Serotonin (5-HT) and the serotonergic system have recently been indicated as modulators of adult hippocampal neurogenesis. In this study, we evaluated the role of 5-HT on the functional features in neurospheres derived from adult neural stem cells (ANSC). We cultured neurospheres derived from mouse hippocampus in serum-free medium containing epidermal (EGF) and type-2 fibroblast growth factor (FGF2). Under these conditions ANSC expressed both isoforms of tryptophane-hydroxylase (TPH) and produced 5-HT. Blocking TPH function by para-chlorophenylalanine (PCPA) reduced ANSC proliferation, which was rescued by exogenous 5-HT. 5-HT action on ANSC was mediated predominantly by the serotonin receptor subtype 5-HT1A and, to a lesser extent, through the 5-HT2C (receptor) subtype, as shown by selectively antagonizing these receptors. Finally, we documented a 5-HT-induced increase of ANSC migration activity. In summary, we demonstrated a powerful serotonergic impact on ANSC functional features, which was mainly mediated by 5-HT1A receptors. Neuropsychopharmacology (2010) 35, 893-903; doi:10.1038/npp.2009.181; published online 9 December 2009
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据