期刊
NEUROPHARMACOLOGY
卷 62, 期 4, 页码 1671-1683出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2011.11.013
关键词
Acetylcholine; Eserine; Carbachol; Muscarinic receptor knockout mice; EPSCs
资金
- National Institute of Mental Health [MH-072908]
- Yerkes National Primate Research Center [RR-00165]
- National Institutes of Health
The anterolateral cell group of the bed nucleus of the stria terminalis (BNSTALG) serves as an important relay station in stress circuitry. Limbic inputs to the BNSTALG are primarily glutamatergic and activity-dependent changes in this input have been implicated in abnormal behaviors associated with chronic stress and addiction. Significantly, local infusion of acetylcholine (ACh) receptor agonists into the BNST trigger stress-like cardiovascular responses, however, little is known about the effects of these agents on glutamatergic transmission in the BNSTALG. Here, we show that glutamate- and ACh-containing fibers are found in close association in the BNSTALG. Moreover, in the presence of the acetylcholinesterase inhibitor, eserine, endogenous ACh release evoked a long-lasting reduction of the amplitude of stimulus-evoked EPSCs. This effect was mimicked by exogenous application of the ACh analog, carbachol, which caused a reversible, dose-dependent, reduction of the evoked EPSC amplitude, and an increase in both the paired-pulse ratio and coefficient of variation, suggesting a presynaptic site of action. Uncoupling of postsynaptic G-proteins with intracellular GDP-beta-S, or application of the nicotinic receptor antagonist, tubocurarine, failed to block the carbachol effect. In contrast, the carbachol effect was blocked by prior application of atropine or M-2 receptor-preferring antagonists, and was absent in M-2/M-4 receptor knockout mice, suggesting that presynaptic M-2 receptors mediate the effect of ACh. Immunoelectron microscopy studies further revealed the presence of M-2 receptors on axon terminals that formed asymmetric synapses with BNST neurons. Our findings suggest that presynaptic M-2 receptors might be an important modulator of the stress circuit and hence a novel target for drug development. (c) 2011 Elsevier Ltd. All rights reserved.
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