期刊
NEUROPHARMACOLOGY
卷 61, 期 8, 页码 1265-1274出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2011.07.029
关键词
Na+ channel; Na(V)1.7; Insulin-like growth factor-1; Glycogen synthase kinase-3 beta; Up-regulation; Chromaffin cell; Catecholamine secretion
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [60295227, 90506833]
Insulin-like growth factor-1 (IGF-1) plays important roles in the regulation of neuronal development. The electrical activity of Na+ channels is crucial for the regulation of synaptic formation and maintenance/repair of neuronal circuits. Here, we examined the effects of chronic IGF-1 treatment on cell surface expression and function of Na+ channels. In cultured bovine adrenal chromaffin cells expressing Na(V)1.7 isoform of voltage-dependent Na+ channels, chronic IGF-1 treatment increased cell surface [H-3]saxitoxin binding by 31%, without altering the Kd value. In cells treated with IGF-1, veratridine-induced Na-22(+) influx, and subsequent Ca-45(2+) influx and catecholamine secretion were augmented by 35%, 33%, 31%, respectively. Pharmacological properties of Na+ channels characterized by neurotoxins were similar between nontreated and IGF-1-treated cells. IGF-1-induced up-regulation of [H-3]saxitoxin binding was prevented by phosphatydil inositol-3 kinase inhibitors (LY204002 or wortmannin), or Akt inhibitor (Akt inhibitor IV). Glycogen synthase kinase-3 (GSK-3) inhibitors (LiCl, valproic acid, SB216763 or SB415286) also increased cell surface [H-3]saxitoxin binding by similar to 33%, whereas simultaneous treatment of IGF-1 with GSK-3 inhibitors did not produce additive increasing effect on [H-3]saxitoxin binding. IGF-1 (100 nM) increased Ser(437)-phosphorylated Akt and Ser(9)-phosphorylated GSK-3 beta, and inhibited GSK-3 beta activity. Treatment with IGF-1, LiCl or SB216763 increased protein level of Na+ channel alpha-subunit; it was prevented by cycloheximide. Either treatment increased alpha-subunit mRNA level by similar to 48% and accelerated alpha-subunit gene transcription by similar to 30% without altering alpha-subunit mRNA stability. Thus, inhibition of GSK-3 beta caused by IGF-1 up-regulates cell surface expression of functional Na+ channels via acceleration of alpha-subunit gene transcription. (C) 2011 Elsevier Ltd. All rights reserved.
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