期刊
NEURON
卷 83, 期 1, 页码 69-86出版社
CELL PRESS
DOI: 10.1016/j.neuron.2014.05.035
关键词
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资金
- CFAR [5P30 AI028697]
- NIH [5R37 MH060233, 5R01 MH094714, K99MH102357, T32MH073526]
- Broad Stem Cell Research Center (BSCRC)
- Autism Speaks
- California Institute for Regenerative Medicine (CIRM)-BSCRC Training Grant [TG2-01169]
- Dr. Miriam and Sheldon Adelson Medical Foundation
Neural stem cells have been adopted to model a wide range of neuropsychiatric conditions in vitro. However, how well such models correspond to in vivo brain has not been evaluated in an unbiased, comprehensive manner. We used transcriptomic analyses to compare in vitro systems to developing human fetal brain and observed strong conservation of in vivo gene expression and network architecture in differentiating primary human neural progenitor cells (phNPCs). Conserved modules are enriched in genes associated with ASD, supporting the utility of phNPCs for studying neuropsychiatric disease. We also developed and validated a machine learning approach called CoNTExT that identifies the developmental maturity and regional identity of in vitro models. We observed strong differences between in vitro models, including hiPSC-derived neural progenitors from multiple laboratories. This work provides a systems biology framework for evaluating in vitro systems and supports their value in studying the molecular mechanisms of human neurodevelopmental disease.
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