期刊
NEURON
卷 78, 期 4, 页码 714-728出版社
CELL PRESS
DOI: 10.1016/j.neuron.2013.03.011
关键词
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资金
- MRC UK
- BBSRC UK [BB/E023223/1]
- Biotechnology and Biological Sciences Research Council [BB/E023223/1, BB/E022553/1] Funding Source: researchfish
- Medical Research Council [MC_U105170643] Funding Source: researchfish
- BBSRC [BB/E022553/1, BB/E023223/1] Funding Source: UKRI
- MRC [MC_U105170643] Funding Source: UKRI
The role of intracellular transcriptional/post-translational feedback loops (TTFL) within the circadian pacemaker of the suprachiasmatic nucleus (SCN) is well established. In contrast, contributions from G-coupled pathways and cytosolic rhythms to the intercellular control of SCN pacemaking are poorly understood. We therefore combined viral transduction of SCN slices with fluorescence/bioluminescence imaging to visualize GCaMP3-reported circadian oscillations of intracellular calcium [Ca2+](i) alongside activation of Ca2+/cAMP-responsive elements. We phase-mapped them to the TTFL, in time and SCN space, and demonstrated their dependence upon G-coupled vasoactive intestinal peptide (VIP) signaling. Pharmacogenetic manipulation revealed the individual contributions of Gq, Gs, and Gi to cytosolic and TTFL circadian rhythms. Importantly, activation of Gq-dependent (but not Gs or Gi) pathways in a minority of neurons reprogrammed [Ca2+](i) and TTFL rhythms across the entire SCN. This reprogramming was mediated by intrinsic VIPergic signaling, thus revealing a Gq/[Ca2+](i)-VIP leitmotif and unanticipated plasticity within network encoding of SCN circadian time.
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