期刊
NEURON
卷 76, 期 3, 页码 549-564出版社
CELL PRESS
DOI: 10.1016/j.neuron.2012.07.027
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [18200025, 20019013, 21220008, 22650070, 40365226, 23110002]
- Core Research for Evolutional Science and Technology from the Japanese Science and Technology Agency
- Grants-in-Aid for Scientific Research [21220008, 22650070, 20019013, 23500469, 18200025] Funding Source: KAKEN
Differentiation of pre- and postsynaptic sites is coordinated by reciprocal interaction across synaptic clefts. At parallel fiber (PF)-Purkinje cell (PC) synapses, dendritic spines are autonomously formed without PF influence. However, little is known about how presynaptic structural changes are induced and how they lead to differentiation of mature synapses. Here, we show that Cbln1 released from PFs induces dynamic structural changes in PFs by a mechanism that depends on postsynaptic glutamate receptor delta2 (GluD2) and presynaptic neurexin (Nrx). Time-lapse imaging in organotypic culture and ultrastructural analyses in vivo revealed that Nrx-Cbln1-GluD2 signaling induces PF protrusions that often formed circular structures and encapsulated PC spines. Such structural changes in PFs were associated with the accumulation of synaptic vesicles and GluD2, leading to formation of mature synapses. Thus, PF protrusions triggered by Nrx-Cbln1-GluD2 signaling may promote bidirectional maturation of PF-PC synapses by a positive feedback mechanism.
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