期刊
NEURON
卷 69, 期 2, 页码 287-303出版社
CELL PRESS
DOI: 10.1016/j.neuron.2010.12.024
关键词
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资金
- National Institutes of Health [MH070860]
- CIHR [MOP-84241, MOP-12675]
- Canada Research Chair
- Michael Smith Foundation for Health Research
- Japan Society for the Promotion of Science
Neurotrophin receptor tyrosine kinases (Trks) have well-defined trophic roles in nervous system development through kinase activation by neurotrophins. Yet Trks have typical cell-adhesion domains and express noncatalytic isoforms, suggesting additional functions. Here we discovered noncatalytic TrkC in an unbiased hippocampal neuron-fibroblast coculture screen for proteins that trigger differentiation of neurotransmitter release sites in axons. All TrkC isoforms, but not TrkA or TrkB, function directly in excitatory glutamatergic synaptic adhesion by neurotrophin-independent high-affinity trans binding to axonal protein tyrosine phosphatase receptor PTP sigma. PTP sigma triggers and TrkC mediates clustering of postsynaptic molecules in dendrites, indicating bidirectional synaptic organizing functions. Effects of a TrkC-neutralizing antibody that blocks TrkC-PTP sigma interaction and TrkC knockdown in culture and in vivo reveal essential roles of TrkC-PTP sigma in glutamatergic synapse formation. Thus, postsynaptic TrkC trans interaction with presynaptic PTP sigma generates bidirectional adhesion and recruitment essential for excitatory synapse development and positions these signaling molecules at the center of synaptic pathways.
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