期刊
NEURON
卷 66, 期 1, 页码 85-100出版社
CELL PRESS
DOI: 10.1016/j.neuron.2010.03.028
关键词
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资金
- National Institutes of Health [R01 DC02281, RR-15301]
- Klingenstein Award
- National Science Foundation [MCB080015]
- Department of Energy [DE-AC02-06CH11357]
- US Department of Energy
- Helen Hay Whitney Foundation
- Howard Hughes Medical Institute
The hair-cell tip link, a fine filament directly conveying force to mechanosensitive transduction channels, is composed of two proteins, protocadherin-15 and cadherin-23, whose mutation causes deafness. However, their molecular structure, elasticity, and deafness-related structural defects are unknown. We present crystal structures of the first and second extracellular cadherin repeats of cadherin-23. Overall, structures show typical cadherin folds, but reveal an elongated N terminus that precludes classical cadherin interactions and contributes to an N-terminal Ca2+-binding site. The deafness mutation D101G, in the linker region between the repeats, causes a slight bend between repeats and decreases Ca2+ affinity. Molecular dynamics simulations suggest that cadherin-23 repeats are stiff and that either removing Ca2+ or mutating Ca2+-binding residues reduces rigidity and unfolding strength. The structures define an uncharacterized cadherin family and, with simulations, suggest mechanisms underlying inherited deafness and how cadherin-23 may bind with itself and with protocadherin-15 to form the tip link.
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