期刊
NEURON
卷 62, 期 2, 页码 254-268出版社
CELL PRESS
DOI: 10.1016/j.neuron.2009.02.027
关键词
-
资金
- National Research Service Award (NRSA)
- postdoctoral fellowship from the National Institutes of Health (NIH)
- National Institute of Mental Health (NIMH)
- Max Planck Society and EUSynapse
- American Heart Association
The precise subunit composition of synaptic ionotropic receptors in the brain is poorly understood. This information is of particular importance with regard to AMPA-type glutamate receptors, the multimeric complexes assembled from GluA1-A4 subunits, as the trafficking of these receptors into and out of synapses is proposed to depend upon the subunit composition of the receptor. We report a molecular quantification of synaptic AMPA receptors (AMPARs) by employing a single-cell genetic approach coupled with electrophysiology in hippocampal CA1 pyramidal neurons. In contrast to prevailing views, we find that GIuA1A2 heteromers are the dominant AMPARs at CA1 cell synapses (similar to 80%). In cells lacking GluA1, -A2, and -A3, synapses are devoid of AMPARs, yet synaptic NMDA receptors (NMDARs) and dendritic morphology remain unchanged. These data demonstrate a functional dissociation of AMPARs from trafficking of NMDARs and neuronal morphogenesis. This study provides a functional quantification of the subunit composition of AMPARs in the CNS and suggests novel roles for AMPAR subunits in receptor trafficking.
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