4.0 Article

Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid

期刊

NEUROIMMUNOMODULATION
卷 25, 期 5-6, 页码 280-284

出版社

KARGER
DOI: 10.1159/000491395

关键词

Autism spectrum disorder; Valproic acid; Lymph nodes

资金

  1. Brazilian National Institute of Science and Technology on Neuroimmunomodulation (INCT-NIM)
  2. Rio de Janeiro
  3. National Council of Scientific and Technological Development (CNPq)
  4. Brazilian Coordination for the Improvement of Higher Education Personnel (CAPES)
  5. Rio de Janeiro State Foundation for Funding Research (Faperj)
  6. Oswaldo Cruz Foundation (Fiocruz)
  7. Clinical Hospital of Porto Alegre (FIPE-HCPA)
  8. Fund for Structural Convergence (FOCEM) of MercoSur

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Objective: Considering the potential role of lymphocytes in the pathophysiology of autism spectrum disorder (ASD), we aimed to evaluate possible alterations of T cell pools in the lymphoid organs of an animal model of autism induced by valproic acid (VPA). Pregnant Swiss mice received a single intraperitoneal injection of 600 mg/kg of VPA (VPA group) or saline (control group) on day 11 of gestation. Male offspring were euthanized on postnatal day 60 for removal of thymuses, spleens, and a pool of inguinal, axillary and brachial lymph nodes. Cellularity was evaluated, and flow cytometry analysis was performed on cell suspensions incubated with the mouse antibodies anti-CD3-FITC, anti-CD4-PE, and anti-CD8-PE-Cy7. We observed that the prenatal exposure to VPA induced a reduction in the numbers of CD3+CD4+ T cells in their lymph nodes when compared to the control animals. This was specific since it was not seen in the thymus or spleen. The consistent decrease in the number of CD4+ T cells in subcutaneous lymph nodes of mice from the animal model of autism may be related to the allergic symptoms frequently observed in ASD. Further research is necessary to characterize the immunological patterns in ASD and the connection with the pathophysiology of this disorder. (C) 2018 S. Karger AG, Basel

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