期刊
NEUROIMAGE
卷 66, 期 -, 页码 634-641出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.neuroimage.2012.10.074
关键词
Optogenetics; fMRI; Heating; Artefact; MRI; Opto-fMRI
资金
- Medical Research Council [MR/J013110/1]
- Medical Research Council (MRC Capacity Building Studentship)
- British Heart Foundation
- MRC [MR/J013110/1] Funding Source: UKRI
- Medical Research Council [MR/J013110/1, 1030105] Funding Source: researchfish
The combination of optogenetics and functional magnetic resonance imaging (fMRI) is referred to as opto-fMRI. Optogenetics utilises genetic engineering to introduce light sensitive actuator proteins into cells. Functional MRI (fMRI) is a specialist form of magnetic resonance imaging concerned with imaging changes in blood flow and oxygenation, linked to regional variation in metabolic activity, in the brain. This study describes a methodological concern regarding the effects of light delivery into the brain for the purposes of opto-fMRI. We show that blue light delivery to the naive rat brain causes profound fMRI responses, despite the absence of optogenetic activation. We demonstrate that these fMRI responses are dependent upon laser power and show that the laser causes significant heating. We identify how heating impacts upon the MR signal causing NMR frequency shifts,, and T1 and T2* changes. This study brings attention to a possible confounder which must be taken into account when opto-fMRI experiments are designed. (C) 2012 Elsevier Inc. All rights reserved.
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