期刊
NEUROCHEMISTRY INTERNATIONAL
卷 63, 期 5, 页码 522-532出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2013.08.008
关键词
Flavonoid-rich extract; Rosa laevigata Michx; Free radicals; Cerebral ischemia reperfusion; Apoptosis; Neuroinflammation
资金
- Program for Liaoning Innovative Research Team in University (LNIRT)
- fund of National Science and Technology Major Special Project on Major New Drug Innovation [2012ZX09503001-003]
- funds of Science Foundation of Science & Technology Bureau of Liaoning Province [2009225009-2, 2012225020]
- fund of Liaoning Province Education Administration [L20133452]
The neuroprotective effect and mechanism of the flavonoid-rich extract (FRE) from Rosa laevigata Michx fruit on cerebral ischemia reperfusion (I/R) injury were investigated. The contents of flavonoids, saponins and tannin were determined, and ten chemicals including chlorogenic acid, 4-hydroxy-3-methoxybenzoic acid, apigenin, luteolin, kaempferol, querce-tin, kaempferide-3-O-glucoside, quercetin-3-rhamnoside, rutin and isorhamnetin-3-O-beta-rutinoside from the crude extract were separated. Oral administration of FRE obviously improved the survival rate and prevented I/R-induced disability and histological damage. Further works showed that the natural product had excellent antioxidant activity, significantly decreased DNA fragmentation, up-regulated the expression of Bcl-2, and down-regulated the expressions of p53, Apaf1, Fas, FasL, Bax, Bid, cytochrome C and active Caspase-3, -9 and -8. Moreover, the FRE decreased the expressions of NF-kappa B, iNOS, MMP-9, COX-2, TNF-alpha, IL-1 beta, IL-4, IL-6, and down-regulated the levels of p-JNK, p-ERK and p-p38 in MAPK pathways. Therefore, the flavonoid-rich extract from R. laevigata Michx fruit has the potential actions for treatment of ischemic stroke due to its anti-oxidant, anti-apoptosis and anti-inflammatory properties. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据