期刊
NEUROCHEMISTRY INTERNATIONAL
卷 61, 期 8, 页码 1424-1432出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2012.10.009
关键词
Aging; Brain energy metabolism; Amino acids; Mitochondria types; CDP; Choline
资金
- Italian Ministry of Instruction, University and Research (M.I.U.R.)
The effect of aging and CDP-choline treatment (20 mg kg(-1) body weight i.p. for 28 days) on the maximal rates (V-max) of representative mitochondrial enzyme activities related to Krebs' cycle (citrate synthase, alpha-ketoglutarate dehydrogenase, malate dehydrogenase), glutamate and related amino acid metabolism (glutamate dehydrogenase, glutamate-oxaloacetate- and glutamate-pyruvate transaminases) were evaluated in non-synaptic and intra-synaptic light and heavy mitochondria from frontal cerebral cortex of male Wistar rats aged 4, 12, 18 and 24 months. During aging, enzyme activities vary in a complex way respect to the type of mitochondria, i.e. non-synaptic and intra-synaptic. This micro-heterogeneity is an important factor, because energy-related mitochondrial enzyme catalytic properties cause metabolic modifications of physiopathological significance in cerebral tissue in vivo, also discriminating pre- and post-synaptic sites of action for drugs and affecting tissue responsiveness to noxious stimuli. Results show that CDP-choline in vivo treatment enhances cerebral energy metabolism selectively at 18 months, specifically modifying enzyme catalytic activities in non-synaptic and intra-synaptic light mitochondrial sub-populations. This confirms that the observed changes in enzyme catalytic activities during aging reflect the bioenergetic state at each single age and the corresponding energy requirements, further proving that in vivo drug treatment is able to interfere with the neuronal energy metabolism. (C) 2012 Published by Elsevier Ltd.
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