期刊
NEUROCHEMISTRY INTERNATIONAL
卷 61, 期 7, 页码 1192-1201出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2012.08.012
关键词
Resveratrol; Alzheimer's disease; Amyloid beta; Inhibitor; Surface plasmon resonance
资金
- National Key Project for Basic Research of the Ministry of Science and Technology of China [2011CB504100]
- Key Program of Natural Science Foundation of Anhui Provincial Educational Commission of China [KJ2010A185]
As currently understood, Alzheimer's disease (AD) is a chronic neurodegenerative disorder that is driven by the aggregation of amyloid beta (A beta) protein. It has been shown that resveratrol (RES) may attenuate amyloid D peptide-induced toxicity, promote AD clearance and reduce senile plaques. However, it remains to be determined whether RES could interact directly with AD. The aim of the present study was to examine the direct binding of RES to monomer and fibril AD. Using surface plasmon resonance (SPR) and proton nuclear magnetic resonance (H-1 NMR), our results identified the direct binding of RES to AD. The ability of RES to bind to both fibril and monomer A beta(1-40 and 1-42) was further analyzed by SPR. The binding response of RES to fA beta(1-42) was higher than that to monomer A beta(1-42), whereas the binding response of RES to fA beta(1-40) was lower than that to monomer A beta(1-40). The K-D of RES for fibril A beta(1-40 or 1-42) was higher than that for the corresponding monomer AD. Compared to the control compound Congo red (CR), the binding responses of RES to monomer A beta(1-42) and A beta(1-40) were stronger, but binding to fibril A beta(1-42) was weaker, and the K(D)s of RES with both monomer and fibril N beta(1-40) and A beta(1-42) were higher than that of CR. When A beta(1-40 or 1-42) was co-incubated with RES (50 ISM), the thioflavin T fluorescence of the mixture was weakened, and the number and length of amyloid fibrils were decreased. Furthermore, the results of staining in consecutive brain slices from AD patients showed that RES (10(-4) M) could stain senile plaques. These results indicated that RES could bind directly to A beta in different states, which may provide new insight into the protective properties of RES against AD. (C) 2012 Elsevier Ltd. All rights reserved.
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