期刊
NEUROCHEMISTRY INTERNATIONAL
卷 59, 期 1, 页码 90-96出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2011.05.016
关键词
Nitric oxide; Diffusion; Brain; In vivo; Microelectrode; Half-life
资金
- FCT (Portugal) [SAU-BEB/103228/2008, PTDC/SAU-NEU/103538/2008]
- Calouste Gulbenkian Foundation
- FCT [SFRH/BD/31051/2006, SFRH/BD/27333/2006]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/31051/2006, SFRH/BD/27333/2006, PTDC/SAU-NEU/103538/2008] Funding Source: FCT
Nitric oxide (No-center dot) is a diffusible messenger that conveys information based on its concentration dynamics, which is dictated by the interplay between its synthesis, inactivation and diffusion. Here, we characterized (NO)-N-center dot diffusion in the rat brain in vivo. By direct sub-second measurement of (NO)-N-center dot, we determined the diffusion coefficient of (NO)-N-center dot in the rat brain cortex. The value of 2.2 x 10(-5) cm(2)/s obtained in vivo was only 14% lower than that obtained in agarose gel (used to evaluate (NO)-N-center dot free diffusion). These results reinforce the view of (NO)-N-center dot as a fast diffusing messenger but, noticeably, the data indicates that neither (NO)-N-center dot diffusion through the brain extracellular space nor homogeneous diffusion in the tissue through brain cells can account for the similarity between (NO)-N-center dot free diffusion coefficient and that obtained in the brain. Overall, the results support that (NO)-N-center dot diffusion in brain tissue is heterogeneous, pointing to the existence of a pathway that facilitates (NO)-N-center dot diffusion, such as cell membranes and other hydrophobic structures. (C) 2011 Elsevier B.V. All rights reserved,
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