期刊
NEUROCHEMICAL RESEARCH
卷 38, 期 8, 页码 1553-1560出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-013-1055-7
关键词
Parkinson's disease; Inflammation; Rotenone; NF kappa B
资金
- National Natural Science Foundation of China [81274122, 81102831, 81073078]
- Special Purpose for New Drug Development [2012ZX093 01002-004]
- Studies on Structure and function of Bioactive Substances from Natural Medicines [IRT1007]
- National Natural Science Foundation of Beijing [7131013]
- Research Fund for the Doctoral Program of Higher Education of China [20121106130001]
Parkinson's disease (PD) is a common neurodegenerative disease, and its etiology remains obscure. Increasing evidence has suggested an important role for environmental factors such as exposure to pesticides in increasing the risk of developing PD and inflammation is the early incident during the process of PD. In this study, we measure the pro-inflammatory cytokines by enzyme-linked immunosorbnent assay and RT-PCR methods; analyze the reactive oxygen species by DCFH-DA; detected nuclear factor kappa B (NF kappa B) translocation by western blot and immunofluorescence methods; and analyze the phosphorylation of mitogen-activated protein (MAP) kinase and protein level of Nurr1 by western blot. Results showed that rotenone could induce tumor neurosis factor alpha (TNF alpha) and interleukin 1 beta (IL-1 beta) release from BV-2 cells, enhance TNF alpha and IL-1 beta mRNA levels in substantia nigra lesioned by rotenone; also, rotenone could increase the phosphorylation of inhibitor of kappa B (I kappa B), extracellular regulated protein kinase , c-Jun N-terminal kinase, p38 MAP kinases and promote p65 subunit of NF kappa B translocation to nuclear; at the same time, rotenone could decrease the protein level of Nurr1 in nuclear. So, rotenone exerted toxicity through activating microglia, and its mechanism might be associated with NF kappa B signal pathway.
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