期刊
NEUROCHEMICAL RESEARCH
卷 37, 期 4, 页码 771-777出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-011-0671-3
关键词
Tenuigenin; Hippocampal neural stem cells; Proliferation; Differentiation
资金
- National Natural Science Foundation of China [81102624, 30973513]
- Beijing Natural Science Foundation [7102073]
- Beijing Key Subject Discipline of Integrated Traditional and Western Medicine [VI 26]
The present study was to investigate the influence of tenuigenin, an active ingredient of Polygala tenuifolia Willd, on the proliferation and differentiation of hippocampal neural stem cells in vitro. Tenuigenin was added to a neurosphere culture and neurosphere growth was measured using MTT assay. The influence of tenuigenin on the proliferation of neural progenitors was examined by Clone forming assay and BrdU detection. In addition, the differentiation of neural stem cells was compared using immunocytochemistry for beta III-tubulin and GFAP. The results showed that addition of tenuigenin to the neural stem cell medium increased the number of newly formed neuro-spheres. More neurons were also obtained when tenuigenin was added in the differentiation medium. These findings suggest that tenuigenin is involved in regulating the proliferation and differentiation of hippocampal neural stem cells. This result may be one of the underlying reasons for tenuigenin's nootropic and anti-aging effects.
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