期刊
NEUROCHEMICAL RESEARCH
卷 36, 期 1, 页码 58-66出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-010-0263-7
关键词
Guillain-Barre syndrome; Campylobacter jejuni; DNA-binding protein from starved cells; Axonal degeneration; Node of Ranvier; Sulfatide
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Ministry of Health, Labour and Welfare of Japan
- grant for Research on Brain Science
Campylobacter jejuni (C. jejuni) is frequently associated with axonal Guillain-Barre syndrome (GBS). We reported that C. jejuni DNA-binding protein from starved cells (C-Dps) binds to and damages myelinated nerves in vivo. We studied the binding patterns of C-Dps to nervous tissues and its in vitro effects on neural cells. Immunohistochemically, C-Dps labeled the nodes of Ranvier, the outermost parts of internodal myelin and the basement membrane in the peripheral nerves, and neurons and myelin in the central nervous tissues. Its binding was blocked by sulfatide. C-Dps bound to the cell surfaces of nerve growth factor (NGF)-treated PC12 cells leading to dose-dependent LDH release, which was inhibited by either heat-denaturation of C-Dps or coincubation with an anti-C-Dps mAb. However, its binding to the surfaces of cultured NSC34 cells, S16 cells, or dorsal root ganglion cells, did not induce cytotoxicity. These findings suggest a possible involvement of C-Dps in C. jejuni-related GBS.
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