期刊
NEUROBIOLOGY OF LEARNING AND MEMORY
卷 109, 期 -, 页码 160-168出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.nlm.2014.01.011
关键词
Fragile X premutation; CGG KI mouse; Fmrp; Arc; c-Fos; Western blot; Endophenotype
资金
- National Institute of Health (NIH) [NINDS R01 NS079775, NINDS RL1 NS062411]
- Roadmap Initiative Grant from NIDCR [UL1 DE019583]
- NIH Neuroscience Training Grant [T32 MH 82174]
- NCRR [UL1 RR024146]
- NeuroTherapeutics Research Institute (NTRI) consortium
Environmental enrichment results in increased levels of Fmrp in brain and increased dendritic complexity. The present experiment evaluated activity-dependent increases in Fmrp levels in the motor cortex in response to training on a skilled forelimb reaching task in the CGG RI mouse model of the fragile X premutation. Fmrp, Arc, and c-Fos protein levels were quantified by Western blot in the contralateral motor cortex of mice following training to reach for sucrose pellets with a non-preferred paw and compared to levels in the ipsilateral motor cortex. After training, all mice showed increases in Fmrp, Arc, and c-Fos protein levels in the contralateral compared to the ipsilateral hemisphere; however, the increase in CGG RI mice was less than wildtype mice. Increases in Fmrp and Arc proteins scaled with learning, whereas this relationship was not observed with the c-Fos levels. These data suggest the possibility that reduced levels of activity-dependent proteins associated with synaptic plasticity such as Fmrp and Arc may contribute to the neurocognitive phenotype reported in the CGG KI mice and the fragile X premutation. (C) 2014 Elsevier Inc. All rights reserved.
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