期刊
NEUROBIOLOGY OF DISEASE
卷 45, 期 3, 页码 954-961出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.nbd.2011.12.014
关键词
Excitotoxicity; Ischemia; Neuronal damage; P2X7
资金
- CIBERNED
- Gobierno Vasco
- Universidad del Pais Vasco
- SK-VEGA [2/0146/09]
Overactivation of subtype P2X7 receptors can induce excitotoxic neuronal death by calcium (Ca2+) overload. In this study, we characterize the functional properties of P2X7 receptors using electrophysiology and Ca2+ monitoring in primary cortical neuron cultures and in brain slices. Both electrical responses and Ca2+ influx induced by ATP and benzoyl-ATP were reduced by Brilliant Blue G (BBG) at concentrations which specifically inhibit P2X7 receptors. In turn, oxygen-glucose deprivation (OGD) caused neuronal death that was reduced with BBC application. OGD in neuron cultures and brain slices generated an inward current, which was delayed and reduced by BOG. To assess the relevance of these in vitro findings, we used middle cerebral artery occlusion in rats as a model of transient focal cerebral ischemia to study the neuroprotective effect of BOG in vivo. Treatment with BBG (twice per day, 30 mg/kg) produced a 60% reduction in the extent of brain damage compared to treatment with vehicle alone. These results show that P2X7 purinergic receptors mediate tissue damage after OGD in neurons and following transient brain ischemia. Therefore, these receptors are a relevant molecular target for the development of new treatments to attenuate brain damage following stroke. (C) 2011 Elsevier Inc. All rights reserved.
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