期刊
NEUROBIOLOGY OF DISEASE
卷 45, 期 1, 页码 288-296出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.nbd.2011.08.012
关键词
Y5 neuropeptide Y receptor; Adeno-associated viral vectors; Kainic acid seizures; Gene therapy
资金
- Swedish Research Council
- EU Commission [LSH-037315]
- Mock Foundation
- Segerfalk Foundation
- Crafoord Foundation
- Elsa Schmitz Foundation
- Swedish Brain Foundation
- Danish Research Council for Health and Disease [64750]
- Lundbeck Foundation
- Foundation of Dr. Sofus Carl Emil Friis and his wife Olga Doris Friis
- Elsass Foundation
- Sophus Jacobsen and his wife Astrid Jacobsen's Foundation
- Jacob Madsen and his wife Olga Madsen Foundation
- Aase and Ejnar Danielsen's Foundation
- Hede Nielsen Family Foundation
- Ivan Nielsen Foundation
- Danish Research Foundation
We recently demonstrated that recombinant adeno-associated viral vector-induced hippocampal overexpression of neuropeptide Y receptor, Y2, exerts a seizure-suppressant effect in kindling and kainate-induced models of epilepsy in rats. Interestingly, additional overexpression of neuropeptide Y in the hippocampus strengthened the seizure-suppressant effect of transgene Y2 receptors. Here we show for the first time that another neuropeptide Y receptor, Y5, can also be overexpressed in the hippocampus. However, unlike Y2 receptor overexpression, transgene Y5 receptors in the hippocampus had no effect on kainate-induced motor seizures in rats. However, combined overexpression of Y5 receptors and neuropeptide Y exerted prominent suppression of seizures. This seizure-suppressant effect of combination gene therapy with Y5 receptors and neuropeptide Y was significantly stronger as compared to neuropeptide Y overexpression alone. These results suggest that overexpression of Y5 receptors in combination with neuropeptide Y could be an alternative approach for more effective suppression of hippocampal seizures. (C) 2011 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据