期刊
NEUROBIOLOGY OF DISEASE
卷 36, 期 2, 页码 393-400出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.nbd.2009.08.011
关键词
DJ-1 mRNA; Parkinson's disease; Quantitative real-time PCR; Post-mortem human brain
资金
- PDS UK
- PD Spring UK
- Reta Lila Weston Institute
- Medical Research Council [G0200373, G0701075] Funding Source: researchfish
- Parkinson's UK [G-0907] Funding Source: researchfish
- MRC [G0701075, G0200373] Funding Source: UKRI
Mutations in the DJ-1 gene have been linked with rare cases of early onset, autosomal recessive Parkinson's disease (PD). To determine whether DJ-1 is also involved in the pathogenesis of common forms of PD we have compared DJ-1 mRNA levels in a number of post-mortem PD and control brain regions using quantitative real-time PCR. Region-specific decreases were observed in DJ-1 mRNA levels in putamen, frontal cortex, parietal cortex and cerebellum in PD (similar to 30-60%) compared to controls whilst an up-regulation was observed in the amygdala (similar to 90%) and entorhinal cortex (similar to 39%). Using quantitative western blot analysis, parallel decreases in DJ-1 protein levels were seen in frontal cortex, putamen and cerebellum of PD cases. By using 2-dimensional gel electrophoresis, we show preponderance of acidic pI isoforms of DJ-1 monomer in PD vulnerable regions, namely frontal cortex and medulla suggestive of differential post-translational modifications. Our findings point to a putative role of DJ-1 in the pathogenesis of PD. (C) 2009 Elsevier Inc. All rights reserved.
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