期刊
NEUROBIOLOGY OF AGING
卷 34, 期 10, 页码 2341-2351出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2013.04.010
关键词
Excitatory synapse; Inhibitory synapse; Glutamate decarboxylase; Astrocytes; Amyloid plaque; Alzheimer's disease
资金
- Australian National Health and Medical Research Council
- J.O. and J.R. Wicking Trust (ANZ Charitable Services)
Amyloid-beta (A beta) plaque accumulation in Alzheimer's disease (AD) is associated with glutamatergic synapse loss, but less is known about its effect on inhibitory synapses. Here, we demonstrate that vesicular gamma-aminobutyric acid (GABA) transporter (VGAT) presynaptic bouton density is unaffected in human preclinical and end-stage AD and in APP/PS1 transgenic (TG) mice. Conversely, excitatory vesicular glutamate transporter 1 (VGlut1) boutons are significantly reduced in end-stage AD cases and less reduced in preclinical AD cases and TGs. Aged TGs also show reduced protein levels of VGlut1 and synaptophysin but not VGAT or glutamate decarboxylase (GAD). These findings indicate that GABAergic synapses are preserved in human AD and mouse TGs. Synaptosomes isolated from plaque-rich TG cortex had significantly higher GAD activity than those from plaque-free cerebellum or the cortex of wild-type littermates. Using tissue fractionation, this increased activity was localized to glial synaptosomes, suggesting that A beta plaques stimulate increased astrocyte GABA synthesis. (C) 2013 Elsevier Inc. All rights reserved.
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